alexa fluor 405 alexa fluor a647 activator reporter dye pair98  (Cell Signaling Technology Inc)

Journal: Cell

Article Title: Differential pre-malignant programs and microenvironment chart distinct paths to malignancy in human colorectal polyps

doi: 10.1016/j.cell.2021.11.031

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: p-STAT3, D3A7 clone, A647 dye, antibody , Cell Signaling , Catalog: 4324S; RRID: AB_10694637.

Techniques: Plasmid Preparation, Software

Journal: Cell

Article Title: Differential pre-malignant programs and microenvironment chart distinct paths to malignancy in human colorectal polyps

doi: 10.1016/j.cell.2021.11.031

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: CD20, D-10 clone, A647 dye, antibody , Santa Cruz , Catalog: sc-393894 AF647.

Techniques: Plasmid Preparation, Software

Journal: Matrix Biology Plus

Article Title: Uncovering mediators of collagen degradation in the tumor microenvironment

doi: 10.1016/j.mbplus.2022.100101

Figure Lengend Snippet: CAFs and MR + TAMs are the main cell types in collagen internalization. (A-C) Quantification of collagen internalization by cell types of the tumor microenvironment: Single-cell suspensions of LL/2 tumors (A), LL/2-GFP tumors (B) or EO771.LMB-GFP tumors (C) were cultured overnight with soluble 10 µg/mL A647-labeled collagen type I. Flow cytometry analysis was then performed to determine the internalized fluorescence in CAFs, TAMs, dendritic cells, granulocytes, monocytes, cancer cells, CD45 − CD31 − FAP − cells and CD45 − CD31 − FAP − GFP − cells. n = 2–6. Error bars = SEM. (D) Representative density plots from flow cytometry analysis of LL/2 tumor showing how TAMs were divided into subpopulations negative and positive for MR. Gates were based on isotype control. (E) Representative overlay of histograms from flowcytometry analysis of LL/2 tumors showing the level of internalized collagen in TAMs negative and positive for MR. (F-G) Quantification of collagen internalization by MR + macrophages, MR − macrophages and FAP + fibroblasts from LL/2 (F) and EO771.LMB tumors (G). n = 3–5, except CAFs in 3F ( n = 2). Error bars = SEM. * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001; one-way ANOVA with post hoc Tukey’s multiple comparisons test.

Article Snippet: A vial of Alexa Fluor 647 (A647) fluorescent dye (Life Technologies) dissolved in 0.5 mL PBS was immediately added and incubated with gentle shaking for two hours at room temperature.

Techniques: Cell Culture, Labeling, Flow Cytometry, Fluorescence